Radioiodination of Enterotoxin from Clostridium perfringens Type A using Chloramine T
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چکیده
منابع مشابه
Purification and biochemical properties of Clostridium perfringens type A enterotoxin.
The sporulation-specific enterotoxin of Clostridium perfringens type A, which is the toxin active in human food poisoning, has been purified from extracts of sporulating cells. Highly purified enterotoxin was obtained by treatment of crude cell extract with ribonuclease for 30 min, followed by sequential chromatography on Sephadex G-100, Cellex T cellulose, and hydroxylapatite. Recovery was 65 ...
متن کاملProteolysis of Clostridium perfringens type A enterotoxin during purification.
The small satellite bands of enterotoxin frequently seen in polyacrylamide gels following purification of Clostridium perfringens enterotoxin were found to be due to endogenous protease activity and were not present if phenylmethylsulfonyl fluoride (PMSF; 1 mM) and EDTA (10 mM) were used in the purification protocol. The use of PMSF was avoided by passing gel filtration-purified enterotoxin mat...
متن کاملAssay methods for Clostridium perfringens type A enterotoxin.
Enterotoxin produced by a sporulating culture of Clostridium perfringens type A NCTC 8798 was purified to a level of 3,500 mouse mean lethal doses per mg of nitrogen. High-titer sera were obtained from rabbits injected with enterotoxin and used to compare the sensitivity of serological tests and bioassays for C. perfringens enterotoxin. Reversed passive hemagglutination was by far the most sens...
متن کاملAntibody against Clostridium perfringens type A enterotoxin in human sera.
Antibody against Clostridium perfringens type A enterotoxin was found in 82% of Brazilian and 65% of American serum samples.
متن کاملClostridium perfringens enterotoxin: a brief review.
Ussing chambers were mounted with stripped rabbit ileal mucosa and either crude or pure toxin A added to the mucosal side of the chamber to final concentrations of 800 and 30pg/ml respectively; increases in short-circuit currents of the order of 25pA were observed in both preparations over a period of 90min, at which point the tissue was reponsive to theophylline but not glucose. Histology show...
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ژورنال
عنوان ژورنال: Journal of Applied Bacteriology
سال: 1980
ISSN: 0021-8847
DOI: 10.1111/j.1365-2672.1980.tb01228.x